Vas Taq Blue Master Mix (2X)
Code  BN0200-0125
Classification  PCR試劑 & 1-Step Kit
Size  1.25ml
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VAS Taq Blue Master Mix

Cat. No. : BN0200-0125
Concentration : 2X
Volume : 1.25 ml
Storage : -20 ℃
 
Description
  The Taq master MIx Blue is a ready-to-use, 2X concentrated premix that contains all the reagents (except primers and template) need for running PCR. Simply add primers、template and water to successfully carry out PCR. Taq master MIx Blue offers several advantages. Set up operation and time are significantly reduced. The chance of contaminating component stocks is eliminated. Reduction of reagent handling steps leads to better reproducibility. An inert blue dye and a stabilizer are also present to allow direct loading of the final products on to a gel for analysis.
   
Content
  2X Taq master MIx containing:
  • Taq DNA polymerase
  • Inert blue dye and a stabilizer
  • dNTP mix including dATP、dCTP、dGTP、dTTP
  • 3 mM MgCl2
   
General reaction mixture for PCR
(total 25μl)
 
  1. Set up each reaction as follows:
    Component Volume/ reaction Final conc.
    Taq master mix 12.5 μl 1X
    Forward Primer variable 0. 2~1.0μM
    Reversed Primer variable 0. 2~1.0μM
    Template DNA <1μg  
    H2O up to 25.0 μl  
  2. Mix the solution thoroughly by pipetting up and
    down.
  3. Program the thermal cycler according to the manufactures instructions.
  4. Place the tubes in the thermal cycler and start
    the reaction.
   
Optimized Cycling Protocol
 
Initial activation step: 1 min 95℃
3-step cycling
Denaturation:
0.5 min 94℃
Annealing: 0.5 min 50~68℃
Extension: 1 min(1kb) 72℃
Number of cycles: 35~40  
Final extension: 3 min 72℃
   
PCR performance test
  Activity of PCR is confirmed by using 103 copies plasmid as the template (amplified fragment: 450 bp).