Cat. No. : BN0200-0125
Concentration : 2X
Volume : 1.25 ml
Storage : -20 ℃ |
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Description |
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The Taq master MIx Blue is a ready-to-use, 2X concentrated premix that contains all the reagents (except primers and template) need for running PCR. Simply add primers、template and water to successfully carry out PCR. Taq master MIx Blue offers several advantages. Set up operation and time are significantly reduced. The chance of contaminating component stocks is eliminated. Reduction of reagent handling steps leads to better reproducibility. An inert blue dye and a stabilizer are also present to allow direct loading of the final products on to a gel for analysis. |
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Content |
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2X Taq master MIx containing:
- Taq DNA polymerase
- Inert blue dye and a stabilizer
- dNTP mix including dATP、dCTP、dGTP、dTTP
- 3 mM MgCl2
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General reaction mixture for PCR
(total 25μl) |
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- Set up each reaction as follows:
Component |
Volume/ reaction |
Final conc. |
Taq master mix |
12.5 μl |
1X |
Forward Primer |
variable |
0. 2~1.0μM |
Reversed Primer |
variable |
0. 2~1.0μM |
Template DNA |
<1μg |
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H2O |
up to 25.0 μl |
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- Mix the solution thoroughly by pipetting up and
down.
- Program the thermal cycler according to the manufactures instructions.
- Place the tubes in the thermal cycler and start
the reaction.
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Optimized Cycling Protocol |
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Initial activation step: |
1 min |
95℃ |
3-step cycling
Denaturation: |
0.5 min |
94℃ |
Annealing: |
0.5 min |
50~68℃ |
Extension: |
1 min(1kb) |
72℃ |
Number of cycles: |
35~40 |
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Final extension: |
3 min |
72℃ |
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PCR performance test |
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Activity of PCR is confirmed by using 103 copies plasmid as the template (amplified fragment: 450 bp). |
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