Vas Taq Blue DNA Polymerase Code  BN0190-0250 Classification  PCR試劑 & 1-Step Kit Size  250u Order  Print  PDF  PRODUCT DETAIL VAS Taq Blue Cat. No. : BN0190-0250 units : 250 U Concentration : 5 units/μl Volume : 50 μl Storage : -20 ℃   Supplied form   20mM Tris-HCl pH8.0, 50％glycerol, 100mM KCl, 0.1Mm EDTA, 1mM DTT, 0.5％Tween20, 0.5％NP-40     Description   VAS Taq is isolated and purified from an E.Coli. strain that carries the cloned DNA polymerase gene from Thermus aquqticus YT-1strain.An inert blue dye is added.     Purity(SDS-PAGE)＞99％     10X VAS Taq PCR Buffer   Volume: 1ml / vial Composition: Tris-HCl, KCl, (NH4)2SO4, 15mM MgCl2, pH8.3 .     Unit definition   One unit is the amount of enzyme that will incorporate 10 nmoles of dNTPs into acid-insoluble products at 72 in 30 ℃ min.     Primer-mix   Hexamers primer-mix for random annealing at 30°C     PCR performance test   Good performance of DNA amplification by PCR is confirmed by using 2 U Taq will amplified 2.5 kb DNA fragment in a 50μl reaction volume.     General reaction mixture for PCR ( total 50μl )   Taq (5 units/μl ) 0.5μl 10X Taq Buffer 5μl dNTPs(2.5mM) 4μl Template ＜ 1μg Primer 1 0.2~1.0μM Primer 2 0.2~1.0μM H2O upto 50μl     PCR products   As most PCR product amplified with VAS Taq have one A added at 3`- terminus, the obtained PCR product can be directly used for cloning into T-vector.