| 2.0 Master Mix Kit (1.5mM MgCl2) |
| Cat. No.: 140301 (100 Reactions) |
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Cat. No.
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Size
Reactions
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Taq DNA Polymerase Master Mixes
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MgCl2 Conc.
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120301
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100
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1.1x Master Mix
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1.5 mM
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120303
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500
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1.1x Master Mix
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1.5 mM
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120306
|
2500
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1.1x Master Mix
|
1.5 mM
|
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130301
|
100
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1.1x Master Mix
|
2.0 mM
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130303
|
500
|
1.1x Master Mix
|
2.0 mM
|
|
130306
|
2500
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1.1x Master Mix
|
2.0 mM
|
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140301
|
100
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2.0x Master Mix
|
1.5 mM
|
|
140303
|
500
|
2.0x Master Mix
|
1.5 mM
|
|
140306
|
2500
|
2.0x Master Mix
|
1.5 mM
|
|
150301
|
100
|
2.0x Master Mix
|
2.0 mM
|
|
150303
|
500
|
2.0x Master Mix
|
2.0 mM
|
|
150306
|
2500
|
2.0x Master Mix
|
2.0 mM
|
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| Store at -20°C. Reagent for in-vitro laboratory use only |
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| General Description |
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Taq DNA Polymerase Master Mix is a ready-to-use 2.0X reaction mix. Simply add primers, template, and water to successfully carry out primer extensions and other molecular biology applications.
Ampliqon Taq DNA Polymerase, the NH4+ buffer system, dNTPs and magnesium chloride are present in Taq DNA Polymerase Master Mix. Each reaction requires 25 μL of the 2.0X reaction mix. Simply add primers, template and water to a total reaction volume of 50 μL.
Taq DNA Polymerase Master Mix offers several advantages. Set up time is significantly reduced. The chance of contaminating component stocks is eliminated. Reduction of reagent handling steps leads to better reproducibility. Standard tests can be set up with the confidence that results will be consistent every time.
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| Composition of 2.0X Taq Master Mix |
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- 150 mM Tris-HCl pH 8.5, 40 mM (NH4)2S04, 3.0 or 4.0 mM MgCl2*, 0.2% Tween 20®
- 0.4 mM dNTPs
- 0.05 units/μL Ampliqon Taq polymerase
- Stabilizer
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*Taq DNA Polymerase Master Mixes are offered in two final MgCl2 concentrations: 1.5mM and 2.0mM.
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| Suggested Protocol using Taq Master Mix |
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This protocol serves as a guideline for primer exten-sions. Optimal reaction conditions such as incubation times, temperatures, and amount of template DNA may vary and must be individually determined.
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Notes: |
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- Set up reaction mixtures in an area separate from that used for DNA preparation or product analysis.
- The table below shows the reaction set up for a final volume of 50 μL. If desired, the reaction size may be scaled down. Use 10 μL of the 2.0X master mix in a final volume of 20 μL.
- Important: Spin Taq Master Mix vials briefly before use.
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- Set up each reaction as follows:
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| Component |
Vol./reaction |
Final Conc. |
| Taq Master Mix |
25 μL |
1X |
| Primer A |
Variable |
0.1–1.0 μM |
| Primer B |
Variable |
0.1–1.0 μM |
| Distilled Water |
Variable |
- - - - |
| Template DNA |
Variable |
Variable |
| TOTAL volume |
50 μL |
- - - - |
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- Mix gently by pipetting the solution up and down a few times.
- Program the thermal cycler according to the manufacturer´s instructions.
For maximum yield and specificity, temperatures and cycling times should be optimized for each new template target or primer pair.
- Place the tubes in the thermal cycler and start the reaction.
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| Related Products |
| |
|
Description
|
Cat. No.
|
Taq DNA Polymerase (500 Units)
with 10X Ammonium Reaction Buffer
with 10X Standard Reaction Buffer |
110303
|
Taq DNA Polymerase (500 Units)
with 10X Combination Buffer |
110403
|
Taq DNA Polymerase (500 Units)
with 10X Mg++ Free Ammonium Buffer |
110503
|
Taq DNA Polymerase 2.0X Master Mix (100 Reac)
with 2.0 mM MgCl2 |
150301
|
Taq DNA Polymerase 2,0X MaMi RED (100 Reac)
with 1.5 mM MgCl2 |
180301
|
Taq DNA Polymerase 2.0X MaMi RED (100 Reac)
with 2.0 mM MgCl2 |
190301
|
| AccuPOL DNA Polymerase (500 Units) |
210303
|
TEMPase Hot Start DNA Polymerase (500Units)
with 10X TEMPase Buffer I
with 10X TEMPase Buffer II |
220303
|
| UniPOL –Long Range PCR (100 Reac) |
270701
|
| Rapid Ligation Kit (50 React) |
750300
|
| RT-PCR One Tube (100 Reac) |
740301
|
TEMPase Hot Start 2X Master Mix
with TEMPase Buffer I (100 Reac) |
230301
|
TEMPase Hot Start 2X Master Mix
with TEMPase Buffer II (100 Reac) |
230701
|
dNTP Mix (2 x 500μl)
(12.5 mM of each dA, dC, dG and dT) |
501004
|
dNTP Mix, (2 x 500 μl)
(10 mM of each dA, dC, dG and dT), |
502004
|
GC5 Value Efficiency, 108 Cfu/μg pUC19
Chemically Competent Cells, (10x 200μl) |
812010
|
GC5 High Efficiency, 109 Cfu/μg pUC19
Chemically Competent Cells, (10x 50μl) |
805010
|
GC5 High Efficiency, 109 Cfu/μg pUC19
Chemically Competent Cells, (5x 200μl) |
802005
|
SuperPath GC10, 1010 Cfu/μg pUC19
ElectroCompetent Cells, (5x 80μl) |
830805
|
| SOC Medium, 10x 10mL |
800000
|
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Tween 20® is a registered trademark of ICI Americas, Inc. |
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NOTICE |
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In certain countries, patents cover the PCR process. This product is intended for researchers having a license to perform PCR or those not required to obtain a license.
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