|
Cat. No.
|
Size
Reactions
|
Taq DNA Polymerase
Master Mixes
|
MgCl2
Conc.
|
|
AMP120301
|
100
|
1.1x Master Mix
|
1.5 mM
|
|
AMP120303
|
500
|
1.1x Master Mix
|
1.5 mM
|
|
AMP120306
|
2,500
|
1.1x Master Mix
|
1.5 mM
|
|
AMP130301
|
100
|
1.1x Master Mix
|
2.0 mM
|
|
AMP130303
|
500
|
1.1x Master Mix
|
2.0 mM
|
|
AMP130306
|
2,500
|
1.1x Master Mix
|
2.0 mM
|
|
AMP140301
|
100
|
2.0x Master Mix
|
1.5 mM
|
|
AMP140303
|
500
|
2.0x Master Mix
|
1.5 mM
|
|
AMP140306
|
2,500
|
2.0x Master Mix
|
1.5 mM
|
|
AMP150301
|
100
|
2.0x Master Mix
|
2.0 mM
|
|
AMP150303
|
500
|
2.0x Master Mix
|
2.0 mM
|
|
AMP150306
|
2,500
|
2.0x Master Mix
|
2.0 mM
|
|
| |
Store at -20°C. FOR RESEARCH USE ON |
| |
| General Description |
| |
Taq DNA Polymerase Master Mix is a ready-to-use 2.0X
reaction mix. Simply add primers, template, and water to
successfully carry out primer extensions and other
molecular biology applications.
Ampliqon Taq DNA Polymerase, the NH4+ buffer system,
dNTPs and magnesium chloride are present in Taq DNA
Polymerase Master Mix. Each reaction requires 25μL of
the 2.0X reaction mix. Simply add primers, template and
water to a total reaction volume of 50 μL.
Taq DNA Polymerase Master Mix offers several
advantages. Set up time is significantly reduced. The
chance of contamination component stocks is eliminated.
Reduction of reagent handling steps leads to better
reproducibility. Standard tests can be set up with the
confidence that results will be consistent every time.
|
| |
|
| Conposition of 2.0X Taq Master Mix |
| |
- 150 mM Tris-HCl ph 8.5, 40 mM (NH4)2SO4, 3.0 or
4.0 mM MgCl2*, 0.2% Tween 20®
- 0.4 mM dNTPs
- 0.05 units/μL Ampliqon Taq polymerase
- Stabilizer
*Taq DNA Polymerase Master Mixes are offered in
two final MgCl2 concentrations: 1.5mM and 2.0mM.
|
| |
|
| Suggested Protocol using Taq Master Mix |
| |
This protocol serves as a guideline for primer extensions.
Optimal reaction conditions such as incubation
times, temperatures, and amount of template DNA may
vary and must be individually determined.
Notes:
- Set up reaction mixtures in an area separate from
that used for DNA preparation or product analysis.
- The table below shows the reaction set up for a final
volume of 50 μL. If desired, the reaction size may be
scaled down. Use 10 μL of the 2.0X master mix in a
final volume of 20 μL.
- Important : Spin Taq Master Mix vials briefly before
use.
|
| |
| |
- Set up each reaction as follows:
| Component |
Vol. / reaction |
Final |
| Taq Master Mix |
25 μL |
1X |
| Primer A |
Variable |
0.1 - 1.0 μM |
| Primer B |
Variable |
0.1 - 1.0 μM |
| Distilled Water |
Variable |
- - - - |
| Template DNA |
Variable |
Variable |
| TOTAL |
50 μL |
- - - - |
- Mix gently by pipetting the solution up and down a
few times.
- Program the thermal cycler according to the
manufacturer’s instructions.
For maximum yield and specificity, temperatures
and cycling times should be optimized for each new
template target or primer pair.
- Place the tubes in the thermal cycler and start the
reaction.
|
| |
