McTaq DNA polymerase
Code  BN0160-0500
Classification  PCR試劑 & 1-Step Kit
Size  500u
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PRODUCT DETAIL

Product name : McTaq DNA polymerase
Catalog number: BN0160-0500
Concentration: 2u/μl, 5u/μl
Storage condition : Store at -20°C


Description
 

Taq DNA polymerase is a thermostable DNA polymerase isolated and purified from E.coli strain that carries a plasmid with cloned Taq DNA polymerase. It is lacks 3’-5’ proofreading activity and there is a 5’-3’ exonuclease activity in the same polypeptide as the DNA polymerase. Taq is designed for use in DNA amplification, primer extension reaction and DNA sequencing.
 
Storage Buffer:
 

50mM Tris-HCl (pH7.9); 0.2mM EDTA; 2mM DTT;0.5% NP-40; 0.5% Tween-20; 50% glycerol
 
10X Reaction Buffer:
 
100mM Tris-HCl ( pH9.0 ); 500mM KCl; 20mM MgCl2; 1% Triton X-100, -010mg/ml BSA
   
Unit Definition:
 
One unit incorporate 10 nmole of dNTP into acid-insoluble material in 30 min at 70°C.
   
Standard PCR Method:
 

For 50μl reaction:
 
Water X μl
10X Reaction Buffer 5μl
2.5mM dNTP 4μl
5-50μM forward primer 1μl
5-50μM reverse primer 1μl
* template DNA 10pg-1μg
Taq enzyme 0.5-2u
Final volume 50 μl
   
 
*The amount of DNA template varies according to complexity of its sequence. In the case of mammalian DNA, up to 1μg is used per reaction. Typical amount of yeast, bacterial, and plasmid DNAs used per reaction are 10ng, 1ng, and 10pg, respectively.