CAS# MC100-022
Storage : -20 °C
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| Synonyms: Ribonuclease I; Pancreatic ribonuclease; Ribonuclease 3’- pyrimidinooligonucleotidohydrolase;
RNAse A; Endoribonuclease I |
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| Product Description |
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A major application for Ribonuclease A (RNAse A) is the removal of RNA from preparations of plasmid DNA. In this
application, the presence of DNAse activity
as an impurity is a concern. The boiling-water bath method used to eliminate contaminating DNase acitivity has proven
unreliable. For this reason, Bio Basic
Inc. developed a proprietary chromatographic preparation method for elimination
of DNase activity. RNAse A is an endoribonuclease that attacks at the 3’
phosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. The
highest activity is exhibited with
single stranded RNA. RNAse A is a single chain polypeptide containing 4
disulfide bridges. In contrast to RNAse B, it is not a glycoprotein.4 RNAse A can
be inhibited by alkylation of His12 or His119, which are present in the active site
of the enzyme. Activators of RNAse A include potassium and sodium salts.
Molecular weight: 113.7 kDa (amino acid sequence)
Extinction coefficient: 2 E1% = 7.0 (280 nm) Isoelectric point:3 pI = 9.6
Optimal temperature: 60 °C (activity range of 15-70 °C) Optimal pH: activity range of 6-10
Inhibitors: ribonuclease inhibitor
The chromatographically purified product is supplied as an essentially salt-free lyophilized powder.
Activity: ³60 Kunitz units/mg protein |
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| Precautions and Disclaimer |
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Precautions and Disclaimer
This product is for R&D use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for
information regarding hazards and
safe handling practices.
Note: RNAse A is stable to both heat and detergents. In addition, it adsorbs strongly to glass. Scrupulous precautions are
necessary to insure that residues
of RNAse A do not cause artifacts in processes requiring intact RNA. |
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| Procedure |
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A major application for RNAse A is the removal of RNA from preparations of plasmid DNA. For this application, DNAse
free RNAse A is used at a final concentration of 10 mg/ml. |
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| Preparation Instructions |
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A stock solution of 10 mg/ml RNAse A could be prepared by dissolving 100mg of
RNAse A in 9.925ml of 0.01M NaOAC (pH 5.2), plus 0.075ml of 1M Tris (not pH
adjusted). Please verify final pH to be neutral. |
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