DNaseI Code  BM0080-2000 Classification  DNA & RNA RELATED PRODUCTS Size  2000u Order  Print  PDF  PRODUCT DETAIL DNaseI Catalog number : BM0080-2000 Size : 2,000u (kunitz,25°C), 1ml Concentration: 2u/ul RNase activity: None-detected Store at -20°C     Description:   DNase I is purified from bovine pancreas that degrades single-stranded or double-stranded DNA to produce 3-hydroxyl oligonucleotides. This enzyme is used in molecular biology techniques like digestion of DNA, in the RNA isolation, nick translation and DNase I footprinting.     Storage buffer:   10mM HEPES (pH7.5) 10mM CaCl2 10mm MgCl2 50% glycerol     10X Reaction Buffer:   400mM Tris-HCl ( pH8.0 ) 100mM MgSO4 10mM CaCl2     Heat Inactivation ( Not for RT ):   Add EDTA solution (pH8.0) to final 2mM, heat at 75°C for 10 min.     DNase I Treatment of RNA for RT-PCR   Add the following components to a sterile, RNase-free tube. Keep the tube on ice during pipetting. RNA in water   1 -8μl       10X RNase-free DNase I buffer   1μl ( 10X composition: 400mM Tris-HCl (pH 8.0), 100mM MgSO4 and 10mM CaCl2.)       RNase-free DNase I   1u/μg RNA Add RNase-free water to 10μl Incubate at 37°C for 30 min. Incubate at 70°C for 5 min to inactivate the DNase. Add the treated RNA to the RT-PCR reaction.