Star-RNA Ladder 0.1-2 Kb ‚1ug/ul
Code  BJ0010-0075
Classification  RNA Marker
Size  75ug
Order 加入購物車
Print 
PRODUCT DETAIL

Product name : Star-RNA Ladder 0.1-2 Kb
Catalog number: BJ0010-0075
Size : 75 µg
Storage : Store at -20°C (for 2 months)
  Store at -80°C (long-term)


Description
 

The 0.1-2 Kb RNA Ladder is suitable for sizing single-stranded RNA fragments from 0.1-2 kb. The ladder is synthesized using in vitro transcription of templates from human and rat genes.

The important features of the ladder are listed below:
‧Consists of 10 RNA fragments ranging in the size from 0.1-2 kb
‧0.3 kb reference band is ~2-fold brighter for easy orientation and band size
 
Specifications
 

Storage Buffer: 10 mM HEPES, pH 7.2; 2 mM EDTA
 
Directions
 

Thaw the 0.1-2 Kb RNA Ladder on ice and mix gently to ensure the ladder is homogenous. Avoid vortexing the ladder.
To avoid staining/destaining steps, you can add RNase-free EtBr solution to a final concentration of 10-50 μg/ml to the ladder.
  Formaldehyde Denaturing Gels
 
  1. Pre-run a horizontal 1.5-2.5% agarose gel (2-3 mm, lane width) containing 6% (w/v) formaldehyde, 20 mM MOPS (pH 7.0), 1 mM EDTA submerged in buffer (20 mM MOPS, pH 7.0; 1 mM EDTA) for 10 minutes at 100 V.
  2. To 3 μg (3 μl) of ladder, add loading buffer (recommended final loading buffer concentration is 20 mM MOPS; pH 7.0, 6% w/v formaldehyde, 31% v/v deionized formamide, 1 mM EDTA, 0.0125% xylene cyanol and 0.0125% bromophenol blue) and mix well.
  3. Denature the ladder in loading buffer at 70°C for 10 minutes. Keep on ice for 1-2 minutes to quench.
  4. Load ladder and your RNA samples on the pre-run gel from Step 1.
  5. Perform electrophoresis at 100 V until the bromophenol blue dye has migrated two-thirds the gel length.
  6. Stain the RNA ladder with:
    • 0.5 μg/ml EtBr solution for 10 minutes and detain the gel in deionized water for 10 minutes OR
    • 1:10000 dilution of SYBRR Green II RNA Gel Stain in TBE for 20-40 minutes. No destaining is required.
  7. Visualize ladder bands on a UV transilluminator (302 nm) and photograph using a gel documentation system. The RNA bands may fade and disappear on prolonged exposure to UV light.