X174 RF DNA/Hae III Fragments are prepared from purified fX174 RF DNA that has been digested to completion with Hae III. They are suitable for sizing linear double-stranded DNA from 72 to 1353 bp. These fragments can be visualized by ethidium bromide staining. The fragments can be radioactively labeled by one of the following methods: (i) Partial exonucleolytic degradation and resynthesis with T4 DNA polymerase.
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