| Description |
| |
Lambda DNA / Hind III Markers is created by digesting lambda DNA with restrictin enzyme Hind III. Lambda DNA / Hind III Markers consists of 8 double strand DNA fragments ranging in sizes from 125 to 23,130 base pairs. |
| |
| Features |
| |
★ Ready-to-use
★ The DNA Marker can be applied directly onto an agarose gel. |
| |
| Supplied Condition |
| |
Marker supplied in loading buffer ( 10mM Tris-HCL (pH 8.0), 10mM EDTA, 0.25% bromophenol blue, 0.25% xylene cyanol , 15% Ficoll 400) for immediate use plus a separate vial of 6x Loading Buffer to add to samples.l |
| |
|
| Storage |
| |
★ At 4 ℃ for periods up to a few weeks.
★ For longer periods store at -20 ℃ |
| |
|
| Concentration |
| |
250 ng / 1μl |
| |
| Applications |
| |
★ For 200 applications |
| |
|
| Recommended Loading |
| |
★ 2.5μL |
| |
|
| |
 |
| |
0.625μg on 0.8%
TAE agarose gel |
| |
|
| Recommendations For Use |
| |
★ Aliquot into small volume for preventing freeze and thaw cycles.
★ The cohesive ends of the 12nt cos site of bacteriophage lambda may anneal and form an additional band. These fragments can be separated by heating at 60 -65 ℃ for 5 minutes and then cooling on ice for 3 minutes. |
| |
|
| Ordering Information |
Cat. No. |
Product Name |
Conc. |
Package size |
Recommended Loading |
No. of Bands |
Range (bp) |
BI0020-0500 |
Lambda Hind III Marker Plus |
250ng/1μL |
500μL /vial |
2.5μL/5mm lane width |
11 |
125-23130bp |
|
| |
|
| |
