| Description |
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Lambda DNA / Hind III Markers is created by digesting lambda DNA with restrictin enzyme Hind III. Lambda DNA / Hind III Markers consists of 8 double strand DNA fragments ranging in sizes from 125 to 23,130 base pairs. |
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| Features |
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★ Ready-to-use
★ The DNA Marker can be applied directly onto an agarose gel. |
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| Supplied Condition |
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Marker supplied in loading buffer ( 10mM Tris-HCL (pH 8.0), 10mM EDTA, 0.25% bromophenol blue, 0.25% xylene cyanol , 15% Ficoll 400) for immediate use plus a separate vial of 6x Loading Buffer to add to samples.l |
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| Storage |
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★ At 4 ℃ for periods up to a few weeks.
★ For longer periods store at -20 ℃ |
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| Concentration |
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250 ng / 1μl |
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| Applications |
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★ For 200 applications |
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| Recommended Loading |
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★ 2.5μL |
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0.625μg on 0.8%
ONESTAR
TAE agarose gel |
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| Recommendations For Use |
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★ Aliquot into small volume for preventing freeze and thaw cycles.
★ The cohesive ends of the 12nt cos site of bacteriophage lambda may anneal and form an additional band. These fragments can be separated by heating at 60 -65 ℃ for 5 minutes and then cooling on ice for 3 minutes. |
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| Ordering Information |
Cat. No. |
Product Name |
Conc. |
Package size |
Recommended Loading |
No. of Bands |
Range (bp) |
BI0010-0500 |
Lambda Hind III Marker |
250ng/1μL |
500μL /vial |
2.5μL/5mm lane width |
8 |
125-23130bp |
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