Using the 1st Strand cDNA Synthesis Kit, RNA is reversely transcribed into single-stranded cDNA (1). In this method, AMV Reverse Transcriptase synthesizes the new cDNA strand at a site(s) determined by the type of primer used: at the 3'-end of the poly (A)-mRNA when Oligo-p (dT)15 is used as a primer, at nonspecific spots along the RNA template when using the Random Primer p(dN)6 , or at a primer-binding site for a sequence-specific primer. The resulting first strand cDNA can then be used as a template for PCR.

AMV Reverse Transcriptase is well suited for the preparation of cDNA for use as a PCR template. Fully active in PCR buffer, AMV Reverse Transcriptase features a high specific activity, ensuring large amounts of template. At 42℃, this enzyme is more efficient than M-MLV Reverse Transcriptase.