Download: Datasheet Sfr303 I (Sac II)

CCGC↓GG

GG↑CGCC

Purified from Streptomyces fradiae 303

Isoschizomer Sac II

Concentration:
10000 units/ml

Activity assay:
Phage λ DNA, SE buffer B, 37℃.

Recommended buffer:
B

Ligation/recutting assay:
After 10-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 10 units of enzyme at 37℃.

Heat inactivation (20 min, 65℃)  :
Yes