Download: Datasheet Sfr274 I (Xho I)

C↓TCGAG

GAGCT↑C

Purified from Streptomyces fradiae 274

Isoschizomer Xho I

Concentration:
50000 units/ml

Activity assay:
Phage λ DNA/PvuI digest, SE buffer B, 37℃.

Recommended buffer:
B

Ligation/recutting assay:
After 10-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of  DNA with 5 units of the enzyme at 37℃.

Heat inactivation (20 min, 65℃):
yes