Download: Datasheet Sfi I

GGCCNNNN↓NGGCC

CCGGN↑NNNNCCGG

Purified from Streptomyces fimbriatus

Concentration:
2000-15000 units/ml

Activity assay:
Adenovirus DNA, SE buffer G, 50℃

Recommended buffer:
G

Ligation/recutting assay:
After 3-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of  DNA with 2 units of the enzyme at 50℃.

Heat inactivation (20 min, 65℃):
No