Download: Datasheet Sbf I (Sse8387 I)

CCTGCA↓GG

GG↑ACGTCC

Purified from Streptomyces species Bf6I

Isoschizomer Sse8387 I

Concentration:
5000 units/ml

Activity assay:
Phage λ DNA, SE buffer B, 37℃

Recommended buffer:
Y

Ligation/recutting assay:
After 5-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of  DNA with 10 units of the enzyme at 37℃.

Heat inactivation:
(20 min, 65℃) - No; (20 min, 80℃) - Yes