Download: Datasheet Sal I

G↓TCGAC

CAGCT­↑G

Purified from Streptomyces albus

Concentration:
20000 units/ml

Activity assay:
Phage λ DNA, SE buffer B, 37℃

Recommended buffer:
B

Ligation/recutting assay:
After 20-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of DNA with 60 units of the enzyme at 37℃

Heat inactivation (20 min, 65℃) :
Yes