Download: Datasheet Pvu II

CAG↓CTG

GTC↑GAC   

Purified from Proteus vulgaris

Concentration:
20-50000 units/ml

Activity assay: Phage λ DNA, SE buffer G, 37℃

Recommended buffer:
G

Ligation/recutting assay:
After 20-fold overdigestion with the enzyme, more than 70% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of  DNA with 40 units of the enzyme at 37℃.

Heat inactivation (20 min, 65℃) :
No