Download: Datasheet Pst I

CTGCA↓G

G↑ACGTC

Purified from E.coli strain that carries Pst I gene from Providencia stuartii

Concentration:
20000-50000 units/ml

Activity assay:
Phage λ DNA, SE buffer O, 37℃

Recommended buffer:
O

Ligation/recutting assay:
After 20-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of  DNA with 60 units of the enzyme at 37℃.

Heat inactivation (20 min, 65℃):
No