Download: Datasheet PspOM I

G↓GGCCC

CCCGG↑C

Purified from Pseudomonas species OM

Concentration:
20-50000 units/ml

Activity assay:
Phage λ DNA/Bam H I digest, SE buffer Y, 37℃

Recommended buffer:
Y

Ligation/recutting assay:
After 40-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 100 units of enzyme at 37℃.

Heat inactivation (20 min, 65℃) :
Yes