Download: Datasheet PspE I (Bst E II)

G↓GTNACC

CCANTG↑G

Purified from Pseudomonas species E

Isoschizomer Bst E II

Concentration:
10000 units/ml

Activity assay:
Phage λ DNA, SE buffer B, 37℃

Recommended buffer:
B

Ligation/recutting assay:
After 10-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of  DNA with 20 units of the enzyme at 37℃.

Heat inactivation (20 min, 65℃):
Yes