Download: Datasheet Apa I
Purified from Acetobacter pasteurianus
Phage λ DNA(dam-)/BamH I digest, SE buffer Y, 37℃
After 20-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut.
Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 40 units of enzyme at 37℃.
Heat inactivation (20 min, 65℃):
Note! Cleavage by Apa I may be blocked by overlapping dcm-methylation