Download: Datasheet Afe I (Eco47 III)
Purified from Alcaligenes faecalis T2774
Isoschizomer Eco47 III
Phage λ DNA/BamHI digest, SE buffer Y, 37℃.
After 10-fold overdigestion with the enzyme, more than 80% of the DNA fragments may be ligated and recut.
Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 10 units of enzyme at 37℃.
Heat inactivation (20 min, 65℃):