Download: Datasheet Acc65
Purified from Acinetobacter calcoaceticus
Phage λ DNA, SE buffer W, 37℃
After 10-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut
Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 30 units of enzyme at 37℃.
Heat inactivation (20 min, 65℃):
Note! Activity may be blocked by overlapping dcm methylation.