Download Datasheet: Acc113 I (Sca I)
Purified from Acetinobacter calcoaceticus 113
Isoschizomer Sca I
Phage λ DNA, SE buffer Y, 37℃
After 5-fold overdigestion with the enzyme, more than 70% of the DNA fragments may be ligated and recut.
Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 10 units of enzyme at 37℃.
Heat inactivation (20 min, 65℃):